Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols Paperback - 2016
by Bradley J. Till; Joanna Jankowicz-Cieslak; Owen A. Huynh
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From the rear cover
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Details
- Title Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
- Author Bradley J. Till; Joanna Jankowicz-Cieslak; Owen A. Huynh
- Binding Paperback
- Pages 38
- Volumes 1
- Language ENG
- Publisher Springer
- Publication date 2016-10-17
- Illustrated Yes
- Features Illustrated
- ISBN 9783319386676 / 3319386670
- Weight 0.18 lbs (0.08 kg)
- Dimensions 9.21 x 6.14 x 0.1 in (23.39 x 15.60 x 0.25 cm)
- Category Science
- Dewey Decimal Code 570.28
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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
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