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Use of Real-Time Rt-PCR
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Use of Real-Time Rt-PCR Paperback - 2025

by N. Vijayalakshmi

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Foot-and Mouth Disease virus (FMDV) is diagnosed by virus isolation test combined with antigen ELISA and recently one step RT-PCR. Use of fluorogenic RT-PCR is getting more and more importance for rapid detection of FMDV from clinical samples that cannot be processed by regular virus isolation tests and antigen ELISA. Quantitative real-time RT-PCR (qRT-PCR) combines Reverse Transcription with PCR amplification and detection of positive amplification using fluorescent probes like TaqMan (R) probe. In the present study, a TaqMan(R) probe based qRT-PCR was standardized and used for detection of FMDV genome from clinical samples including nasal secretions, plasma and oesophago-pharyngeal fluids (probang samples). The initial standardization was made using different serial dilutions of a FMDV isolate OTNN 24/84 (106.8 TCID50) and 102 virus could be identified using the TaqMan(R) qRT-PCR. RNA standards were synthesized in vitro from a plasmid containing 110 base pair of 3D region of a FMDV type O isolate.

Details

  • Title Use of Real-Time Rt-PCR
  • Author N. Vijayalakshmi
  • Binding Paperback
  • Pages 64
  • Volumes 1
  • Language ENG
  • Publisher LAP Lambert Academic Publishing
  • Publication date 2025-03-17
  • ISBN 9786208430412 / 6208430410
  • Weight 0.22 lbs (0.10 kg)
  • Dimensions 9 x 6 x 0.15 in (22.86 x 15.24 x 0.38 cm)
  • Category Science

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USE OF REAL-TIME RT-PCR
Stock photo: cover may vary

USE OF REAL-TIME RT-PCR

by N. VIJAYALAKSHMI

  • New
  • Paperback
Condition
New
Binding
Paperback
ISBN 10 / ISBN 13
9786208430412 / 6208430410
Quantity available
463
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Item price
A$77.74
A$15.60 Delivery to USA

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Paperback. New. New Book; Fast Shipping from UK; Not signed; Not First Edition; N/A
Add to wish list
Item price
A$77.74
A$15.60 Delivery to USA